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  • HOME
  • About
  • Research
    • teams >
      • TEAM 1 PATHOPHYSIOLOGY OF THE INTESTINAL EPITHELIUM
      • ​TEAM 2 PATHOGENESIS AND COMMENSALISM OF ENTEROBACTERIA
      • TEAM 3 INTESTINAL NEURO-IMMUNE INTERACTIONS
      • TEAM 4 GENETICS AND REGULATION OF IRON METABOLISM
      • TEAM 5 ERYTHROFERRONE AND IRON HOMEOSTASIS
      • RESEARCH GROUP ENVIRONMENT & INTESTINAL EPITHELIUM INTERACTIONS -EINSTEIN-
    • Core facilities >
      • DIGESTIVE MODELS
      • ORGANOIDS
    • Publications
  • EDUCATION
    • MASTER
    • THESIS >
      • THESIS TOPICS PROPOSALS
      • THESIS DEFENSES
  • JOBS
    • JOB OFFERS
    • PREPARATION FOR COMPETITION
  • Events
    • IRSD Retreat >
      • 2016
      • 2017
    • Progress Reports
    • IRSD seminars
  • SERVICES
    • SERVICES AND LOGISTICS
  • Highlights
  • Pictures
  • Contacts
    • Contact IRSD
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RESEARCH GROUP
ENVIRONMENT & INTESTINAL EPITHELIUM INTERACTIONS
-EINSTEIN-

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Research Group : Environment & INteSTinal Epithelium INteractions
- from stem cells to associated (Dys)Functions -


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​

AUDREY FERRAND &  FRÉDÉRICK BARREAU

Team Leader

RESEARCH DESCRIPTION

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Members

Postdoctoral fellow

ANIMAL AND CELLULAR MODELS / METHODS

Cellular models :
- Human colorectal fibroblast primocultures
- Human and murine 3D colon organoid primocultures
- Caco-2 cells stabely transfected with Nod2 wt or mutated polymorphism.
- 
Caco-2 cells stabely transfected with 300A or 300T variants for ATG16L1.

Animal models :
- Sub-cutaneous or caecum orthotopic grafting in murine models
- Alterations of the Nod2 pathways (mice KO or mutated for Nod2)
- Alterations of the endoplasmic reticulum stress pathway (mice KO for IL-1 and NOX-1)


​Methods:
- 2D and 3D cell culture
- Animal experimentation
- Protein expression (Western-blot, invalidation ...)
- RT-qPCR
- Capillary Electrophoresis
- Confocal and bi-photon imaging
.

PUBLICATIONS

Identification of an analgesic lipopeptide produced by the probiotic Escherichia coli strain Nissle 1917. Pérez-Berezo T, Pujo J, Martin P, Le Faouder P, Galano JM, Guy A, Knauf C, Tabet JC, Tronnet S, Barreau F, Heuillet M, Dietrich G, Bertrand-Michel J, Durand T, Oswald E, Cenac N. Nat Commun. 2017 Nov 3;8(1):1314. doi: 10.1038/s41467-017-01403-9. PMID: 29101366 Free PMC Article Similar articles

Oral Administration of the Probiotic Strain Escherichia coli Nissle 1917 Reduces Susceptibility to Neuroinflammation and Repairs Experimental Autoimmune Encephalomyelitis-Induced Intestinal Barrier Dysfunction. Secher T, Kassem S, Benamar M, Bernard I, Boury M, Barreau F, Oswald E, Saoudi A. Front Immunol. 2017 Sep 14;8:1096. doi: 10.3389/fimmu.2017.01096. eCollection 2017. PMID:28959254 Free PMC Article Similar articles

Complementary Roles of Nod2 in Hematopoietic and Nonhematopoietic Cells in Preventing Gut Barrier Dysfunction Dependent on MLCK Activity. Al Nabhani Z, Montcuquet N, Roy M, Dussaillant M, Hugot JP, Barreau F. Inflamm Bowel Dis. 2017 Jul;23(7):1109-1119. doi: 10.1097/MIB.0000000000001135. PMID:28520587 Similar articles

.Nod2: The intestinal gate keeper. Al Nabhani Z, Dietrich G, Hugot JP, Barreau F. PLoS Pathog. 2017 Mar 2;13(3):e1006177. doi: 10.1371/journal.ppat.1006177. eCollection 2017 Mar. Review. PMID:28253332 Free PMC Article Similar articles

Anti-inflammatory and anticancer effects of flavonol glycosides from Diplotaxis harra through GSK3β regulation in intestinal cells.
Nasri I, Chawech R, Girardi C, Mas E, Ferrand A, Vergnolle N, Fabre N, Mezghani-Jarraya R, Racaud-Sultan C. Pharm Biol. 2017 Dec;55(1):124-131. Epub 2016 Sep 22. PMID:27925497 Similar articles

Mu and delta opioid receptor knockout mice show increased colonic sensitivity. Reiss D, Ceredig RA, Secher T, Boué J, Barreau F, Dietrich G, Gavériaux-Ruff C. Eur J Pain. 2017 Apr;21(4):623-634. doi: 10.1002/ejp.965. Epub 2016 Oct 17. PMID:27748566 Similar articles

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​CS 60039 31024, TOULOUSE CEDEX 3  
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