RESEARCH GROUP
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Research Group : Environment & INteSTinal Epithelium INteractions
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RESEARCH DESCRIPTION |
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Members
ANIMAL AND CELLULAR MODELS / METHODS
Cellular models :
- Human colorectal fibroblast primocultures
- Human and murine 3D colon organoid primocultures
- Caco-2 cells stabely transfected with Nod2 wt or mutated polymorphism.
- Caco-2 cells stabely transfected with 300A or 300T variants for ATG16L1.
Animal models :
- Sub-cutaneous or caecum orthotopic grafting in murine models
- Alterations of the Nod2 pathways (mice KO or mutated for Nod2)
- Alterations of the endoplasmic reticulum stress pathway (mice KO for IL-1 and NOX-1)
Methods:
- 2D and 3D cell culture
- Animal experimentation
- Protein expression (Western-blot, invalidation ...)
- RT-qPCR
- Capillary Electrophoresis
- Confocal and bi-photon imaging.
- Human colorectal fibroblast primocultures
- Human and murine 3D colon organoid primocultures
- Caco-2 cells stabely transfected with Nod2 wt or mutated polymorphism.
- Caco-2 cells stabely transfected with 300A or 300T variants for ATG16L1.
Animal models :
- Sub-cutaneous or caecum orthotopic grafting in murine models
- Alterations of the Nod2 pathways (mice KO or mutated for Nod2)
- Alterations of the endoplasmic reticulum stress pathway (mice KO for IL-1 and NOX-1)
Methods:
- 2D and 3D cell culture
- Animal experimentation
- Protein expression (Western-blot, invalidation ...)
- RT-qPCR
- Capillary Electrophoresis
- Confocal and bi-photon imaging.
PUBLICATIONS
Identification of an analgesic lipopeptide produced by the probiotic Escherichia coli strain Nissle 1917. Pérez-Berezo T, Pujo J, Martin P, Le Faouder P, Galano JM, Guy A, Knauf C, Tabet JC, Tronnet S, Barreau F, Heuillet M, Dietrich G, Bertrand-Michel J, Durand T, Oswald E, Cenac N. Nat Commun. 2017 Nov 3;8(1):1314. doi: 10.1038/s41467-017-01403-9. PMID: 29101366 Free PMC Article Similar articles
Oral Administration of the Probiotic Strain Escherichia coli Nissle 1917 Reduces Susceptibility to Neuroinflammation and Repairs Experimental Autoimmune Encephalomyelitis-Induced Intestinal Barrier Dysfunction. Secher T, Kassem S, Benamar M, Bernard I, Boury M, Barreau F, Oswald E, Saoudi A. Front Immunol. 2017 Sep 14;8:1096. doi: 10.3389/fimmu.2017.01096. eCollection 2017. PMID:28959254 Free PMC Article Similar articles
Complementary Roles of Nod2 in Hematopoietic and Nonhematopoietic Cells in Preventing Gut Barrier Dysfunction Dependent on MLCK Activity. Al Nabhani Z, Montcuquet N, Roy M, Dussaillant M, Hugot JP, Barreau F. Inflamm Bowel Dis. 2017 Jul;23(7):1109-1119. doi: 10.1097/MIB.0000000000001135. PMID:28520587 Similar articles
.Nod2: The intestinal gate keeper. Al Nabhani Z, Dietrich G, Hugot JP, Barreau F. PLoS Pathog. 2017 Mar 2;13(3):e1006177. doi: 10.1371/journal.ppat.1006177. eCollection 2017 Mar. Review. PMID:28253332 Free PMC Article Similar articles
Anti-inflammatory and anticancer effects of flavonol glycosides from Diplotaxis harra through GSK3β regulation in intestinal cells.
Nasri I, Chawech R, Girardi C, Mas E, Ferrand A, Vergnolle N, Fabre N, Mezghani-Jarraya R, Racaud-Sultan C. Pharm Biol. 2017 Dec;55(1):124-131. Epub 2016 Sep 22. PMID:27925497 Similar articles
Mu and delta opioid receptor knockout mice show increased colonic sensitivity. Reiss D, Ceredig RA, Secher T, Boué J, Barreau F, Dietrich G, Gavériaux-Ruff C. Eur J Pain. 2017 Apr;21(4):623-634. doi: 10.1002/ejp.965. Epub 2016 Oct 17. PMID:27748566 Similar articles
Oral Administration of the Probiotic Strain Escherichia coli Nissle 1917 Reduces Susceptibility to Neuroinflammation and Repairs Experimental Autoimmune Encephalomyelitis-Induced Intestinal Barrier Dysfunction. Secher T, Kassem S, Benamar M, Bernard I, Boury M, Barreau F, Oswald E, Saoudi A. Front Immunol. 2017 Sep 14;8:1096. doi: 10.3389/fimmu.2017.01096. eCollection 2017. PMID:28959254 Free PMC Article Similar articles
Complementary Roles of Nod2 in Hematopoietic and Nonhematopoietic Cells in Preventing Gut Barrier Dysfunction Dependent on MLCK Activity. Al Nabhani Z, Montcuquet N, Roy M, Dussaillant M, Hugot JP, Barreau F. Inflamm Bowel Dis. 2017 Jul;23(7):1109-1119. doi: 10.1097/MIB.0000000000001135. PMID:28520587 Similar articles
.Nod2: The intestinal gate keeper. Al Nabhani Z, Dietrich G, Hugot JP, Barreau F. PLoS Pathog. 2017 Mar 2;13(3):e1006177. doi: 10.1371/journal.ppat.1006177. eCollection 2017 Mar. Review. PMID:28253332 Free PMC Article Similar articles
Anti-inflammatory and anticancer effects of flavonol glycosides from Diplotaxis harra through GSK3β regulation in intestinal cells.
Nasri I, Chawech R, Girardi C, Mas E, Ferrand A, Vergnolle N, Fabre N, Mezghani-Jarraya R, Racaud-Sultan C. Pharm Biol. 2017 Dec;55(1):124-131. Epub 2016 Sep 22. PMID:27925497 Similar articles
Mu and delta opioid receptor knockout mice show increased colonic sensitivity. Reiss D, Ceredig RA, Secher T, Boué J, Barreau F, Dietrich G, Gavériaux-Ruff C. Eur J Pain. 2017 Apr;21(4):623-634. doi: 10.1002/ejp.965. Epub 2016 Oct 17. PMID:27748566 Similar articles
FUNDINGS
