TEAM 1
PATHOPHYSIOLOGY OF THE INTESTINAL  EPITHELIUM

Nathalie Vergnolle

Team Leader

RESEARCH DESCRIPTION

Pathophysiology of the intestinal epithelium

The overall goal of the team "Pathophysiology of the intestinal epithelium" is to understand the mechanisms of diseases associated with dysfunction of the intestinal epithelium: chronic inflammatory bowel disease (IBD), irritable bowel syndrome , celiac disease, colorectal cancer and adenomatous polyposis.
The research team with expertise on the biology of the intestinal mucosa and in particular barrier functions, secretory functions, its absorption, regeneration and the light sensor functions. The role of the proteolytic balance of the mucosa in the intestinal pathologies is more particularly studied.

Part of the team is organized in an independent research group entitled "Interaction between the environment and the intestinal epithelium" under the co-direction of Audrey Ferrand and Frederik Barreau.

Members

Old Members

ANIMAL AND CELLULAR MODELS / METHODS

  • The "organoid" are generated and used as a model for the study of intestinal diseases (IBD Colorectal Cancer) and their treatments. A reconstitutes epithelial organoid body in 3D from stem cells of intestinal tissue. This structure mimics conditions close to reality (molecular and cellular complexity)​
  • culture models of human epithelial cells.
  • Cultures of human submucosal neurons and glial cells.
  • Cultures of sensory neurons.
  • In vitro and in vivo evaluating the barrier function of the epithelium.
  • Animal models of chronic inflammatory bowel disease.
  • Parasitic or bacterial infection models  .
  • Animal models of visceral hypersensitivity.
  • Animal models of colorectal cancer.

PUBLICATIONS

  • Rolland-Fourcade C, Denadai-Souza A, Cirillo C, Lopez C, Jaramillo JO, Desormeaux C, Cenac N, Motta JP, Larauche M, Taché Y, Berghe PV, Neunlist M, Coron E, Kirzin S, Portier G, Bonnet D, Alric L, Vanner S, Deraison C, Vergnolle N. Epithelial expression and function of trypsin-3 in irritable bowel syndrome  Gut. 2017 Oct;66(10):1767-1778
  • Vergnolle, N. Protease inhibition as new therapeutic strategy for GI diseases. Gut 2016 doi:10.1136 / gutjnl-2015-309147
  • Cenac, N., Bautzova, T., Le Faouder P, Veldhuis NA, Poole DP, Rolland C, Bertrand J, Liedtke W, Dubourdeau M, Bertrand-Michel J, Zecchi L, Stanghellini V, Bunnett NW., Barbara, G., Vergnolle, N. Quantification and Potential Functions of Endogenous Agonists of Transient Receptor Potential Channels in Patients With Irritable Bowel Syndrome. Gastroenterology, 2015. Aug;149(2):433-44.e7.
  • Motta, JP., Bermudez-Humaran, L Deraison, C Martin, L., Rolland, C., Rousset, P., Chapman, K., Vinel, JP., Alric, L., Mas, E., Sallenave, JM., Langella, P., Vergnolle, N. Oral food-grade lactic acid bacteria recombinant for Elafin restores elastolytic balance and protects from intestinal inflammation. Science Trans. Med. 2012, 4(158): 158ra-144.
  • Motta, J.P., Magne, L., Descamps, D., Rolland, C., Squarzoni-Dale, C., Rousset, P., Martin, L., Cenac N., Balloy, V., Huerre, M., Jenne, D., Wartelle, J., Belaaouaj, A., Mas, E., Vinel J.P., Alric, L., Chignard, M., Vergnolle, N.*, Sallenave J.M.* Modifying the protease/anti-protease expression pattern by elafin over-expression protects mice from colitis. Gastroenterology 2011, 140(4): 1272-82. * Equal contribution
  • D'Aldebert, E., Cenac, N., Rousset, P., Martin L., Rolland, C., Chapman, K., Selves, J., Alric, L., Vinel, J.P., Vergnolle, N. Function of Transcient Receptor Potential Vanilloid-4 in intestinal epithelial cells: evidences for a pro-inflammatory role. Gastroenterology 2011, 140(1): 275-85.
  • Cenac, N., Altier, C., Motta, J.P., Galeano, S., Zamponi, G.W., Vergnolle, N. Potentiation of TRPV4 signalling by histamine and serotonin: a central mechanism for visceral hypersensitivity. Gut 2010, 59(4): 481-8.
​